Correlation of Serum Magnesium Levels in Type 2 Diabetes Mellitus: An Institutional Study

Introduction: In developing nations, the increased prevalence of type 2 diabetes mellitus (T2DM) has resulted in significant morbidity and socioeconomic consequences. Hypomagnesemia has been associated with insulin resistance and related micro- vascular consequences due to its ability to trigger hyperglycemia. Although many research articles on glycemic control have been published in recent years, the latest therapeutic approaches may not be feasible to all. As a result, prioritising preventative and primary care research becomes critical. Aim and objective: The study is aimed at estimating serum magnesium concentration and glycated hemoglobin (HbA1c) levels in patients with Type 2 Diabetes mellitus. This will help us evaluate how glycemic control in Diabetes can influence serum Magnesium levels. Method: The study consists of 50 consenting patients who came for routine blood investigations. Those patients having high random blood sugar were selected and divided into two groups based on their age. i.e. 25-50, 51-75. Other parameters con- sidered were age, gender and diet. After an overnight fasting, blood of the consenting patient was collected. Magnesium was measured by Cobas 6000 using the calorimeter end-point method. The other blood sample was transferred into an EDTA test tube which was used to estimate HbA1c level. Result: Participants were divided into two groups based on their age. i.e. 25-50, 51-75. The mean serum magnesium levels were 1.46 mg/dL for group 1 and 1.3 mg/dL for group 2. The mean HbA1c levels for group 1 were 7.65 and and 8.36 for group 2 respectively. According to Pearson’s correlation coefficient, inverse correlation was found between HbA1c levels and Serum magnesium levels. Conclusion: Magnesium insufficiency has been linked to a higher incidence of diabetic due to poor glycemic management in people with diabetes. To avoid such problems and maintain glycemic control, dietary supplements may be recommended. Large-scale clinical research is also required.

Comparison of CD105 (Endoglin) Expression in Odontogenic Keratocyst and Dentigerous Cyst - An Immunohistochemistry Study

Introduction: Tumors like Odontogenic Keratocyst (OKC), Dentigerous Cyst (DC)and Pyogenic Granuloma are frequently oc- curring in the oral cavity with each of them having relation to angiogenesis. Higher angiogenesis may be associated with increased tissue metabolism, more aggressive biologic behaviour, and increased recurrence and growth rate. Tumor growth is dependent not only on a rise in the number of blood vessels, but also on factors such as protein molecules produced in en- dothelial cells. Microvessel density (MVD), Microvessel area (MVA), Microvessel perimeter (MVP) can predict the growth of the tumour, metastasis and patient’s survival and this value is related to the aggressiveness of the tumour. Aims: The aim of the present study was to determine the angiogenic potential of OKC and DCcompared with normal mucosa using CD 105 marker immunohistochemically. Materials and methods: Immunohistochemical staining was done on 70 paraffin embedded tissue samples. Histopathologi- cally diagnosed cases of OKC, DC and Pyogenic granuloma and healthy gingival tissue samples were retrieved for the study purpose. Results: There was no statistically significant difference in the mean MVD, MVA, MVP values of OKC, DC and pyogenic granu- loma groups. Conclusion: The angiogenic potential was determined in 3 different cases of OKC, Dentigerous Cyst and Pyogenic granuloma in terms of MVD, MVA and MVP and compared to normal mucosa using CD105 marker immunohistochemically.Though the mean values of MVA, MVD, MVP were statistically not significant but was estimated to be higher than the normal mucosa

Oral Verrucous Hyperplasia with Dysplasia – A Case Report

Introduction: Verrucous Papillary Lesions (VPLs) clinically present themselves as an exophytic growth seen commonly as grey- white in color. A dominant part of the favorable VPLs have viral etiology and incorporate commonly occurring squamous papil- loma alongside verruca vulgaris, central epithelial hyperplasia, and condyloma. Current comprehension of possibly malignant VPLs is astounding and is basically ascribed to the utilization of confounding and unacceptable terminology. Verrucous Hyper- plasia (VH) of the oral mucosa is a relatively intricate entity possessing paradoxical features making it difficult to diagnose when compared to other verruco-papillary lesions such as Verrucous Carcinoma (VC). Case Presentation: This case report depicts the presentation of Oral verrucous hyperplasia along with dysplasia seen in buccal mucosa of a 46-year-old patient. Management and Prognosis: Surgical excision was performed and ensured that margins were included in the excision to avoid any recurrence of the lesion. Conclusion: The most dependable approach to isolate these substances on routine hematoxylin-eosin stained tissue areas is to perceive the exophytic growth patterns of oral verrucous hyperplasia from the combined exophytic and endophytic growth patterns related with verrucous carcinoma. Furthermore, investigations on this are required using Immunohistochemical meth- ods.

Successful medical management of a neonate with spontaneous splenic rupture and severe hemophilia A

Splenic rupture in neonates is a rare event, usually occurring in the setting of underlying predisposing conditions. Here, we present the case of a term neonate who presented with worsening anemia in the setting of known hemolytic disease during the newborn period and was later found to have a spontaneous splenic rupture. He was subsequently diagnosed with severe hemophilia A, and was managed medically with recombinant factor VIII replacement therapy without any surgical intervention. This is the first reported case of a neonate who had spontaneous splenic rupture and severe hemophilia A, and underwent successful medical treatment without any surgical intervention

Nine years’ review on preseptal and orbital cellulitis and emergence of community–acquired methicillin-resistant Staphylococus aureus in a tertiary hospital in India.

Context: Preseptal cellulitis is the commonest orbital disease which frequently needs to be differentiated from orbital cellulitis. Prompt diagnosis and treatment with appropriate antibiotics can prevent vision loss and life-threatening complications of orbital cellulitis. Aims: To describe the clinical profile of cases with preseptal and orbital cellulitis admitted to a tertiary care hospital during a period of nine years. The causative organisms and the clinical outcome were analyzed. Settings and Design: Retrospective descriptive case study done in a tertiary care hospital in South India. Material and Methods: The in-patient records of patients with preseptal and orbital cellulitis were reviewed from 1998 to 2006. The factors reviewed included ocular findings aiding in the distinction of the two clinical conditions, the duration of symptoms, the duration of hospital stay, microbiological culture report of pus or wound swab, blood culture, drugs used for treatment, the response to therapy and complications. Statistical Analysis Used: Descriptive analysis. Results: One hundred and ten cases, 77 patients with preseptal cellulitis and 33 patients with orbital cellulitis were reviewed. Five percent of children and 21% of adults presented with cutaneous anthrax contributing to preseptal cellulitis. Thirty-nine percent cases with orbital cellulitis were caused by methicillin-resistant Staphylococcus aureus (MRSA). Conclusions: This study has helped in identifying organisms which cause orbital infections, especially community-acquired MRSA. It indicates the need for modifying our empirical antimicrobial therapy, especially in orbital cellulitis.

Production of paclitaxel by Fusarium solani isolated from Taxus celebica.

A fungus was isolated from the stem cuttings of Taxus celebica, which produced paclitaxel in liquid-grown cultures. The fungus was identified as Fusarium solani based on colony characteristics, morphology of conidia and the 26S rDNA sequence. Paclitaxel was identified by chromatographic and spectroscopic comparison with authentic paclitaxel and its cytotoxic activity towards Jurkat cells in vitro.

Mechanism of the immunomodulatory activity of glycodelin.

Glycodelin, a progesterone regulated protein synthesized by the endometrium (GdA) has been well documented to inhibit the proliferation of activated T-cells and is an indispensable molecule in the maternal system for the establishment, maintenance and progression of pregnancy. Data from our laboratory have unequivocally shown that the immunosuppression by GdA is via induction of apoptosis in activated T cells. Another isoform of glycodein, GdS, from the male reproductive system, in spite of sharing an identical amino acid sequence as that of GdA has been shown not to harbour the immunosuppressive activity of GdA. As the only difference between the two proteins is glycosylation, we proposed to study the role of the sugars in imparting apoptotic activity to Gd. Using the recombinant baculovirus system, Gd lacking glycosylation was expressed and from the experimental observations we could conclude that the activity of Gd lies in the protein backbone. Recombinant Gd expressed in P. pastoris, and Chinese hamster ovary cells, like the GdS did not exhibit apoptotic activity. A close analyses of the glycans associated with the Gd molecules from various sources suggested that though the apoptogenic activity of Gd lies in the protein backbone, the glycans modulate the activity by masking (as in case of GdS and most recombinant Gd expressed in our laboratory) or unmasking (as in case of GdA and baculovirus expressed Gd), the functional region of the molecule.

Antigenic determinants on chicken riboflavin carrier protein. A study with monoclonal antibodies.

Monoclonal antibodies raised against chicken egg white riboflavin carrier protein were classified into seven categories each recognizing a distinct epitope. Of these, six were directed against conformation dependent epitopes and one to a sequential epitope. The roles of lysine residues and the post-translationally attached phosphate and oligosaccharide moieties in the antigenicity of riboflavin carrier protein recognized by the monoclonal antibodies were investigated. The binding region of three monoclonal antibodies could be located within the 87–219 amino acid sequence of the protein and one antibody among these recognized a sequence of 182–204 amino acid residues. All the monoclonal antibodies were able to recognize riboflavin carrier proteins present in the sera of pregnant rats, cows and humans indicating that the epitopes to which they are directed are conserved through evolution from chicken to the human.